Protein structure determination using X-ray crystallography is the major experimental technique in Structural Biology. This module will provide an in-depth introduction to the technique. No similar module is offered at this level anywhere in South Africa.
Programme Co-ordinator, Trevor Sewell, has experience in computer-based education, and is actively engaged in research in the field of structural biology. In addition to his co-ordinating role, Prof Sewell will present lectures on the determination of the three-dimensional structures of cells using tomography and single particle methods for the determination of macromolecular structure. He will also present workshops explaining some of the major achievements of structural biology studies on large structures including the ribosome.
Senior Lecturer, Muhammed Sayed (Department of Biotechnology, UWC), is a protein crystallographer who completed his post-doctoral studies under Professor Tom Blundell at Cambridge University. He will supervise the web-courses in protein structure and protein crystallography. His goal is to develop protein crystallography as a practical discipline. He will be involved in the "Introduction to X-ray crystallography" sub-module.
International consultant, Mike Lawrence, will also present lectures and workshops on protein crystallography
This module descriptor document also can be downloaded as an MS-Word document.
The ability to:
|Home Department:||Biotechnology (UWC)|
|Module description (Header):||Protein Crystallography|
|Generic module name:||Structural Biology|
|Credit Value:||20 Credits|
|Prerequisites:||Computational and Mathematical Primer|
|Learning time breakdown (hours):|
|Contact with lecturer/tutor:||40|
|Assignments & tasks:||60|
|Tests & examinations:||5|
|Total Learning Time||200|
|Methods of Student Assessment:||Students will be required to present at least two lectures on specialist topics and complete three computer-based assignments demonstrating mastery of a variety of techniques. There will also be a class test. Moderation will be internal.
Lectures - 10%
Class test - 10%
Assignments - 80%
Read the sections on MIR and MAD in the courseware (Birkbeck Macromolecular Structure Determination Course):
Web course located at
[PC] Tutorial 1: X-ray data processing by Mike Lawrence
[PC] Tutorial 2: Phasing by isomorphous replacement by Mike Lawrence