Staff publications

Staff publications

Journal Articles

Crum, MA, JM Park, BT Sewell, and MJ Benedik. 2015. "C-terminal hybrid mutant of Bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization." Journal of Applied Microbiology, 118 (4): 881-889.

Crum, MA, JM Park, AE Mulelu, BT Sewell, and MJ Benedik. 2015. "Probing C-terminal interactions of the Pseudomonas stutzeri cyanide-degrading CynD protein." Applied Microbiology and Biotechnology, 99 (7): 3093-3102.

Ambele, MA, BT Sewell, FR Cummings, PJ Smith, and TJ Egan. 2013. "Synthetic hemozoin (beta-hematin) crystals nucleate at the surface of neutral lipid droplets that control their sizes." Crystal Growth & Design, 13 (10).

Weber, BW, SW Kimani, A Varsani, DA Cowan, R Hunter, GA Venter, JC Gumbart, and BT Sewell. 2013. "The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning." Journal of Biological Chemistry, 288 (40): 28514-28523.

Baum, S, DS Williamson, T Sewell, and A Stolz. 2012. "Conversion of sterically demanding alpha,alpha-disubstituted phenylacetonitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191." Applied and Environmental Microbiology, 78 (1): 48-57.

Broadley, SG, JC Gumbart, BW Weber, MJ Marakalala, DJ Steenkamp, and BT Sewell. 2012. "A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism." Acta Crystallographica. Section D, Biological crystallography, 68 (Pt 11): 1450-1459.

Manole, V, P Laurinmaki, W Van Wyngaardt, CA Potgieter, IM Wright, GJ Venter, AA van Dijk, BT Sewell, and SJ Butcher. 2012. "Structural insight into African horsesickness virus infection." Journal of Virology, 86 (15): 7858-7866.

Twala, BV, BT Sewell, and J Jordaan. 2012. "Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSyn™ polymer microspheres." Enzyme and Microbial Technology, 50 (6-7): 331-336.

Wang, L, JM Watermeyer, AE Mulelu, BT Sewell, and MJ Benedik. 2012. "Engineering pH-tolerant mutants of a cyanide dihydratase." Applied Microbiology and Biotechnology, 94 (1): 131-140.

Chan, KY, J Gumbart, R McGreevy, JM Watermeyer, BT Sewell, and K Schulten. 2011. "Symmetry-restrained flexible fitting for symmetric EM maps." Structure (London, England: 1993), 19 (9): 1211-1218.

Miller, JL, J Woodward, S Chen, M Jaffer, B Weber, K Nagasaki, Y Tomaru, R Wepf, A Roseman, A Varsani, and T Sewell. 2011. "Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy." Journal of General Virology, 92 (8): 1960-1970.

Nel, AJM, IM Tuffin, BT Sewell, and DA Cowan. 2011. "Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate." Applied and Environmental Microbiology, 77 (11): 3696-3702.

van Rooyen, JM, VR Abratt, H Belrhali, and T Sewell. 2011. "Crystal structure of Type III glutamine synthetase: surprising reversal of the inter-ring interface." Structure (London, England: 1993), 19 (4): 471-483.

van Rooyen, J, H Belrhali, V Abratt, and BT Sewell. 2011. "Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements." Acta Crystallographica. Section F, Structural biology and crystallization communications, 67 (Pt 3): 358-363.

Achilonu, I, S Gildenhuys, L Fisher, J Burke, S Fanucchi, BT Sewell, M Fernandes, and HW Dirr. 2010. "The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function." Acta Crystallographica. Section F, Structural biology and crystallization communications, 66 (Pt 7): 776-780.

Briddon, RW, DP Martin, BE Owor, L Donaldson, PG Markham, RS Greber, and A Varsani. 2010. "A novel species of mastrevirus (family Geminiviridae) isolated from Digitaria didactyla grass from Australia." Archives of Virology, 155 (9): 1529-1534.

Briddon, RW, J Heydarnejad, F Khosrowfar, H Massumi, DP Martin, and A Varsani. 2010. "Turnip curly top virus, a highly divergent geminivirus infecting turnip in Iran." Virus Research, 152 (1–2): 169-175.

Erdmann, JB, DN Shepherd, DP Martin, A Varsani, EP Rybicki, and H Jeske. 2010. "Replicative intermediates of maize streak virus found during leaf development." Journal of General Virology, 91 (4): 1077-1081.

Gildenhuys, S, M Dobreva, N Kinsley, Y Sayed, J Burke, S Pelly, GP Gordon, M Sayed, BT Sewell, and HW Dirr. 2010. "Arginine 15 stabilizes an S(N)Ar reaction transition state and the binding of anionic ligands at the active site of human glutathione transferase A1-1." Biophysical Chemistry, 146 (2-3): 118-125.

Joubert, MK, N Kinsley, A Capovilla, BT Sewell, MS Jaffer, and M Khati. 2010. "A modeled structure of an aptamer-gp120 complex provides insight into the mechanism of HIV-1 neutralization." Biochemistry, 49 (28): 5880-5890.

Lefeuvre, P, DP Martin, G Harkins, P Lemey, AJA Gray, S Meredith, F Lakay, A Monjane, J Lett, A Varsani, and J Heydarnejad. 2010. "The spread of tomato yellow leaf curl virus from the Middle East to the world." PLoS Pathog, 6 (10).

Opperman, DJ, BT Sewell, D Litthauer, MN Isupov, JA Littlechild, and E van Heerden. 2010. "Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01." Biochemical and Biophysical Research Communications, 393 (3): 426-431.

Ortiz-Catedral, L, B Kurenbach, M Massaro, K McInnes, DH Brunton, ME Hauber, DP Martin, and A Varsani. 2010. "A new isolate of beak and feather disease virus from endemic wild red-fronted parakeets (Cyanoramphus novaezelandiae) in New Zealand." Archives of Virology, 155 (4): 613-620.

Petersen, LN, S Marineo, S Mandala, F Davids, BT Sewell, and RA Ingle. 2010. "The missing link in plant histidine biosynthesis: Arabidopsis myoinositol monophosphatase-like2 encodes a functional histidinol-phosphate phosphatase." Plant Physiology, 152 (3): 1186-1196.

Shepherd, DN, DP Martin, E van der Walt, K Dent, A Varsani, and EP Rybicki. 2010. "Maize streak virus: an old and complex 'emerging' pathogen." Molecular Plant Pathology, 11 (1): 1-12.

Tazehkand, SA, AH Pour, J Heydarnejad, A Varsani, and H Massumi. 2010. "Identification of phytoplasmas associated with cultivated and ornamental plants in Kerman Province, Iran." Journal of Phytopathology, 158 (11-12): 713-720.

van Rooyen, JM, VR Abratt, H Belrhali, and BT Sewell. 2010. "Crystallization of recombinant Bacteroides fragilis glutamine synthetase (GlnN) isolated using a novel and rapid purification protocol." Protein expression and purification, 74 (2): 211-216.

Varsani, A, GK de Villiers, GL Regnard, RR Bragg, K Kondiah, II Hitzeroth, and EP Rybicki. 2010. "A unique isolate of beak and feather disease virus isolated from budgerigars (Melopsittacus undulatus) in South Africa." Archives of Virology, 155 (3): 435-439.

Watermeyer, JM, WL Kroger, HG O Neill, BT Sewell, and ED Sturrock. 2010. "Characterization of domain-selective inhibitor binding in angiotensin-converting enzyme using a novel derivative of lisinopril." Biochemical Journal, 428 (1): 67-74.

Williamson, DS, KC Dent, BW Weber, A Varsani, J Frederick, RN Thuku, RA Cameron, JH van Heerden, DA Cowan, and BT Sewell. 2010. "Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8." Applied Microbiology and Biotechnology, 88 (1): 143-153.

Woodward, JD and BT Sewell. 2010. "Tomography of asymmetric bulk specimens imaged by scanning electron microscopy." Ultramicroscopy, 110 (2): 170-175.

Dent, KC, BW Weber, MJ Benedik, and BT Sewell. 2009. "The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat." Applied Microbiology and Biotechnology, 82 (2): 271-278.

Frouws, TD, HG Patterton, and BT Sewell. 2009. "Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber." Biophysical Journal, 96 (8): 3363-3371.

Thuku, RN, D Brady, MJ Benedik, and BT Sewell. 2009. "Microbial nitrilases: versatile, spiral forming, industrial enzymes." Journal of Applied Microbiology, 106 (3): 703-727.

Varsani, A, DN Shepherd, K Dent, AL Monjane, EP Rybicki, and DP Martin. 2009. "A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family." Virology Journal, 6.

Woodward, JD, R Wepf, and BT Sewell. 2009. "Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs." Journal of Microscopy, 234 (3): 287-292.

Basile, LJ, RC Wilson, and BT Sewell. 2008. "Genome mining of cyanide-degrading nitrilases from filamentous fungi." Applied Microbiology and Biotechnology, 80 (3): 427-435.

Castillo-Urquiza, GP, JEA Beserra, FP Bruckner, ATM Lima, A Varsani, P Alfenas-Zerbini, and FM Zerbini. 2008. "Six novel begomoviruses infecting tomato and associated weeds in Southeastern Brazil." Archives of Virology, 153 (10): 1985-1989.

Oluwafemi, S, A Varsani, AL Monjane, DN Shepherd, BE Owor, EP Rybicki, and DP Martin. 2008. "A new African streak virus species from Nigeria." Archives of Virology, 153 (7): 1407-1410.

Shepherd, DN, DP Martin, P Lefeuvre, AL Monjane, BE Owor, EP Rybicki, and A Varsani. 2008. "A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue." Journal of Virological Methods, 149 (1): 97-102.

Shepherd, DN, A Varsani, OP Windram, P Lefeuvre, AL Monjane, BE Owor, and DP Martin. 2008. "Novel sugarcane streak and sugarcane streak Reunion mastreviruses from southern Africa and La Réunion." Archives of Virology, 153 (3): 605-609.

van Antwerpen, T, SA McFarlane, GF Buchanan, DN Shepherd, DP Martin, EP Rybicki, and A Varsani. 2008. "First report of Maize streak virus field infection of sugarcane in South Africa." Plant Disease, 92 (6): 982.

van der Walt, E, DP Martin, A Varsani, JE Polston, and EP Rybicki. 2008. "Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias." Virology Journal, 5 (1).

Varsani, A, S Oluwafemi, OP Windram, DN Shepherd, AL Monjane, BE Owor, EP Rybicki, P Lefeuvre, and DP Martin. 2008. "Panicum streak virus diversity is similar to that observed for maize streak virus." Archives of Virology, 153 (3): 601-604.

Varsani, A, DN Shepherd, AL Monjane, BE Owor, JB Erdmann, EP Rybicki, M Peterschmitt, RW Briddon, PG Markham, S Oluwafemi, OP Windram, P Lefeuvre, J Lett, and DP Martin. 2008. "Recombination, decreased host specificity and increased mobility may have driven the emergence of maize streak virus as an agricultural pathogen." Journal of General Virology, 89 (9): 2063-2074.

Watermeyer, JM, WL Kroger, HG O Neill, BT Sewell, and ED Sturrock. 2008. "Probing the basis of domain-dependent inhibition using novel ketone inhibitors of angiotensin-converting enzyme." Biochemistry, 47 (22): 5942-5950.

Windram, OP, B Weber, MA Jaffer, EP Rybicki, DN Shepherd, and A Varsani. 2008. "An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins." Archives of Virology, 153 (3): 585-589.

Woodward, JD, BW Weber, MP Scheffer, MJ Benedik, A Hoenger, and BT Sewell. 2008. "Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi." Journal of Structural Biology, 161 (2): 111-119.

Bredell, H, DP Martin, J van Harmelen, A Varsani, HW Sheppard, R Donovan, CM Gray, and C Williamson. 2007. "HIV type 1 subtype C gag and nef diversity in Southern Africa." AIDS Research and Human Retroviruses, 23 (3): 477-481.

Corradi, HR, I Chitapi, BT Sewell, D Georgiadis, V Dive, ED Sturrock, and KR Acharya. 2007. "The structure of testis angiotensin-converting enzyme in complex with the C domain-specific inhibitor RXPA380." Biochemistry, 46 (18): 5473-5478.

Danilov, SM, JM Watermeyer, IV Balyasnikova, K Gordon, EV Kugaevskaya, YE Elisseeva, RF Albrecht, and ED Sturrock. 2007. "Fine epitope mapping of monoclonal antibody 5F1 reveals anticatalytic activity toward the N domain of human angiotensin-converting enzyme." Biochemistry, 46 (31): 9019-9031.

Kimani, SW, VB Agarkar, DA Cowan, MFR Sayed, and BT Sewell. 2007. "Structure of an aliphatic amidase from Geobacillus pallidus RAPc8." Acta Crystallographica. Section D, Biological Crystallography, 63 (Pt 10): 1048-1058.

Lefeuvre, P, DP Martin, M Hoareau, F Naze, H Delatte, M Thierry, A Varsani, N Becker, B Reynaud, and J-M Lett. 2007. "Begomovirus melting pot in the south-west Indian Ocean islands: molecular diversity and evolution through recombination." Journal of General Virology, 88 (Pt 12): 3458-3468.

Makhongela, HS, AE Glowacka, VB Agarkar, BT Sewell, B Weber, RA Cameron, DA Cowan, and SG Burton. 2007. "A novel thermostable nitrilase superfamily amidase from Geobacillus pallidus showing acyl transfer activity." Applied Microbiology and Biotechnology, 75 (4): 801-811.

Owor, BE, DP Martin, DN Shepherd, Edema, AL Monjane, EP Rybicki, JA Thomson, and A Varsani. 2007. "Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant." Journal of General Virology, 88 (Pt 11): 3154-3165.

Owor, BE, DN Shepherd, NJ Taylor, R Edema, AL Monjane, JA Thomson, DP Martin, and A Varsani. 2007. "Successful application of FTA Classic Card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes." Journal of Virological Methods, 140 (1-2): 100-105.

Papakyriakou, A, GA Spyroulias, ED Sturrock, E Manessi-Zoupa, and P Cordopatis. 2007. "Simulated interactions between angiotensin-converting enzyme and substrate gonadotropin-releasing hormone: novel insights into domain selectivity." Biochemistry, 46 (30): 8753-8765.

Riviere, G, A Michaud, HR Corradi, ED Sturrock, KR Acharya, V Cogez, J-P Bohin, D Vieau, and P Corvol. 2007. "Characterization of the first angiotensin-converting like enzyme in bacteria: Ancestor ACE is already active." Gene, 399 (1): 81-90.

Thuku, RN, BW Weber, A Varsani, and BT Sewell. 2007. "Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form." FEBS Journal, 274 (8): 2099-2108.

Webber, D, AL Rodgers, and ED Sturrock. 2007. "Glycosylation of prothrombin fragment 1 governs calcium oxalate crystal nucleation and aggregation, but not crystal growth." Urological Research, 35 (6): 277-285.

AB, E, DJR Pugh, R Kaptein, R Boelens, and AMJJ Bonvin. 2006. "Direct use of unassigned resonances in NMR structure calculations with proxy residues." Journal of the American Chemical Society, 128 (23): 7566-7571.

Agarkar, VB, SW Kimani, DA Cowan, MFR Sayed, and BT Sewell. 2006. "The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing." Acta crystallographica. Section F, Structural biology and crystallization communications, 62 (Pt 12): 1174-1178.

Corradi, HR, SLU Schwager, AT Nchinda, ED Sturrock, and KR Acharya. 2006. "Crystal structure of the N domain of human somatic angiotensin I-converting enzyme provides a structural basis for domain-specific inhibitor design." Journal of Molecular Biology, 357 (3): 964-974.

Farias, SL, RA Sabatini, TC Sampaio, IY Hirata, MHS Cezari, MA Juliano, ED Sturrock, AK Carmona, and L Juliano. 2006. "Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII." Biological Chemistry, 387 (5): 611-616.

Heath, L, E van der Walt, A Varsani, and DP Martin. 2006. "Recombination patterns in aphthoviruses mirror those found in other picornaviruses." Journal of Virology, 80 (23): 11827-11832.

Kohl, T, I Hitzeroth, D Stewart, A Varsani, VA Govan, ND Christensen, AL Williamson, and EP Rybicki. 2006. "Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge: a proof-of-concept study." Clinical and Vaccine Immunology, 13 (8): 845-853.

Nchinda, AT, K Chibale, P Redelinghuys, and ED Sturrock. 2006. "Synthesis and molecular modeling of a lisinopril-tryptophan analogue inhibitor of angiotensin I-converting enzyme." Bioorganic & Medicinal Chemistry Letters, 16 (17): 4616-4619.

Pugh, DJR, E AB, A Faro, PT Lutya, E Hoffmann, and DJG Rees. 2006. "DWNN, a novel ubiquitin-like domain, implicates RBBP6 in mRNA processing and ubiquitin-like pathways." BMC Structural Biology, 6.

Redelinghuys, P, AT Nchinda, K Chibale, and ED Sturrock. 2006. "Novel ketomethylene inhibitors of angiotensin I-converting enzyme (ACE): inhibition and molecular modelling." Biological Chemistry, 387 (4): 461-466.

Rodgers, AL, PD Mensah, SL Schwager, and ED Sturrock. 2006. "Inhibition of calcium oxalate crystallization by commercial human serum albumin and human urinary albumin isolated from two different race groups: evidence for possible molecular differences." Urological Research, 34 (6): 373-380.

Shepherd, DN, DP Martin, A Varsani, JA Thomson, EP Rybicki, and HH Klump. 2006. "Restoration of native folding of single-stranded DNA sequences through reverse mutations: an indication of a new epigenetic mechanism." Archives of Biochemistry and Biophysics, 453 (1): 108-122.

Tastan Bishop, AO and T Sewell. 2006. "A new approach to possible substrate binding mechanisms for nitrile hydratase." Biochemical and Biophysical Research Communications, 343 (1): 319-325.

van Rooyen, JM, VR Abratt, and BT Sewell. 2006. "Three-dimensional structure of a type III glutamine synthetase by single-particle reconstruction." Journal of Molecular Biology, 361 (4): 796-810.

Varsani, A, AL Williamson, MA Jaffer, and EP Rybicki. 2006. "A deletion and point mutation study of the human papillomavirus type 16 major capsid gene." Virus Research, 122 (1-2): 154-163.

Varsani, A, E van der Walt, L Heath, EP Rybicki, AL Williamson, and DP Martin. 2006. "Evidence of ancient papillomavirus recombination." Journal of General Virology, 87 (Pt 9): 2527-2531.

Varsani, A, A-L Williamson, D Stewart, and EP Rybicki. 2006. "Transient expression of human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector." Virus Research, 120 (1-2): 91-96.

Watermeyer, JM, BT Sewell, SL Schwager, R Natesh, HR Corradi, KR Acharya, and ED Sturrock. 2006. "Structure of testis ACE glycosylation mutants and evidence for conserved domain movement." Biochemistry, 45 (42): 12654-12663.

Webber, D, CM Radcliffe, L Royle, G Tobiasen, AH Merry, AL Rodgers, ED Sturrock, MR Wormald, DJ Harvey, RA Dwek, and PM Rudd. 2006. "Sialylation of urinary prothrombin fragment 1 is implicated as a contributory factor in the risk of calcium oxalate kidney stone formation." FEBS Journal, 273 (13): 3024-3037.

Woodman, ZL, SLU Schwager, P Redelinghuys, AJ Chubb, EL van der Merwe, MRW Ehlers, and ED Sturrock. 2006. "Homologous substitution of ACE C-domain regions with N-domain sequences: effect on processing, shedding, and catalytic properties." Biological Chemistry, 387 (8): 1043-1051.

Balyasnikova, IV, ZL Sun, YV Berestetskaya, AJ Chubb, RF Albrecht, ED Sturrock, and SM Danilov. 2005. "Monoclonal antibodies 1B3 and 5C8 as probes for monitoring the integrity of the C-terminal end of soluble angiotensin-converting enzyme." Hybridoma (2005), 24 (1): 14-26.

Jandhyala, DM, RC Willson, BT Sewell, and MJ Benedik. 2005. "Comparison of cyanide-degrading nitrilases." Applied Microbiology and Biotechnology, 68 (3): 327-335.

Kuhnert, DC, Y Sayed, S Mosebi, M Sayed, BT Sewell, and HW Dirr. 2005. "Tertiary interactions stabilise the C-terminal region of human glutathione transferase A1-1: a crystallographic and calorimetric study." Journal of Molecular Biology, 349 (4): 825-838.

Onyemata, J, M Meyer, JM McKenzie, DJG Rees, and DJR Pugh. 2005. "Investigation of the binding of ceramide and palmitoyl-CoA to murine t-ACBP using heteronuclear NMR spectroscopy." South African Journal of Science, 101 (9-10): 430-434.

Pugh, DJR. 2005. "Biomolecular NMR spectroscopy in South Africa : the first five years." South African Journal of Science, 101 (9-10): 421-429.

Redelinghuys, P, AT Nchinda, and ED Sturrock. 2005. "Development of domain-selective angiotensin I-converting enzyme inhibitors." Annals of the New York Academy of Sciences, 1056, 160-175.

Sewell, BT, RN Thuku, X Zhang, and MJ Benedik. 2005. "Oligomeric structure of nitrilases: effect of mutating interfacial residues on activity." Annals of the New York Academy of Sciences, 1056, 153-159.

Woodman, ZL, SLU Schwager, P Redelinghuys, AK Carmona, MRW Ehlers, and ED Sturrock. 2005. "The N domain of somatic angiotensin-converting enzyme negatively regulates ectodomain shedding and catalytic activity." Biochemical Journal, 389 (Pt 3): 739-744.

Bersanetti, PA, MCC Andrade, DE Casarini, MA Juliano, AT Nchinda, ED Sturrock, L Juliano, and AK Carmona. 2004. "Positional-scanning combinatorial libraries of fluorescence resonance energy transfer peptides for defining substrate specificity of the angiotensin I-converting enzyme and development of selective C-domain substrates." Biochemistry, 43 (50): 15729-15736.

Natesh, R, SLU Schwager, HR Evans, ED Sturrock, and KR Acharya. 2004. "Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme." Biochemistry, 43 (27): 8718-8724.

Sewell, BT, RB Best, S Baum, AM Roseman, GW Farr, AL Horwich, and HR Saibil. 2004. "A mutant chaperonin with rearranged inter-ring electrostatic contacts and temperature-sensitive dissociation." Nature Structural & Molecular Biology, 11 (11): 1128-1133.

Sturrock, ED, R Natesh, JM van Rooyen, and KR Acharya. 2004. "Structure of angiotensin I-converting enzyme." Cellular and Molecular Life Sciences, 61 (21): 2677-2686.

Sturrock, ED, R Natesh, JM van Rooyen, and KR Acharya. 2004. "What's new in the renin-angiotensin system?" Cellular and Molecular Life Sciences, 61 (21): 2677-2686.

Tsekoa, TL, MF Sayed, RA Cameron, BT Sewell, and DA Cowan. 2004. "Purification, crystallization and preliminary X-ray diffraction analysis of thermostable nitrile hydratase." South African Journal of Science, 100 (9-10): 488-491.

Acharya, KR, ED Sturrock, JF Riordan, and MRW Ehlers. 2003. "ACE revisited: a new target for structure-based drug design." Nature Reviews. Drug Discovery, 2 (11): 891-902.

Gordon, K, P Redelinghuys, SLU Schwager, MRW Ehlers, AC Papageorgiou, R Natesh, KR Acharya, and ED Sturrock. 2003. "Deglycosylation, processing and crystallization of human testis angiotensin-converting enzyme." The Biochemical journal, 371 (Pt 2): 437-442.

Guy, JL, RM Jackson, KR Acharya, ED Sturrock, NM Hooper, and AJ Turner. 2003. "Angiotensin-converting enzyme-2 (ACE2): comparative modeling of the active site, specificity requirements, and chloride dependence." Biochemistry, 42 (45): 13185-13192.

Jandhyala, D, M Berman, PR Meyers, BT Sewell, RC Willson, and MJ Benedik. 2003. "CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies." Applied and Environmental Microbiology, 39 (8): 4794-4805.

Sewell, BT, MN Berman, PR Meyers, D Jandhyala, and MJ Benedik. 2003. "The cyanide degrading nitrilase from Pseudomonas stutzeri AK61 is a two-fold symmetric, 14-subunit spiral." Structure (London, England: 1993), 11 (11): 1413-1422.

Varsani, A, A-l Williamson, D de Villiers, I Becker, ND Christensen, and EP Rybicki. 2003. "Chimeric human papillomavirus type 16 (HPV-16) L1 particles presenting the common neutralizing epitope for the L2 minor capsid protein of HPV-6 and HPV-16." Journal of Virology, 77 (15): 8386-8393.

Varsani, A, A-L Williamson, RC Rose, M Jaffer, and EP Rybicki. 2003. "Expression of human papillomavirus type 16 major capsid protein in transgenic Nicotiana tabacum cv. Xanthi." Archives of Virology, 148 (9): 1771-1786.

Webber, D, AL Rodgers, and ED Sturrock. 2003. "Selective inclusion of proteins into urinary calcium oxalate crystals: comparison between stone-prone and stone-free population groups." Journal of Crystal Growth, 259 (1): 179-189.

Egan, TJ, JM Combrinck, J Egan, GR Hearne, HM Marques, S Ntenteni, BT Sewell, PJ Smith, D Taylor, DA van Schalkwyk, and JC Walden. 2002. "Fate of haem iron in the malaria parasite Plasmodium falciparum." Biochemical Journal, 362 (Pt 2): 343-347.

Kirsch, R, MA Jaffer, BE Woodburne, T Sewell, SL Kelly, RE Kirsch, and EG Shephard. 2002. "Fibrinogen is degraded and internalized during incubation with neutrophils, and fibrinogen products localize to electron lucent vesicles." Biochemical Journal, 364 (Pt 2): 403-412.

Nicolls, FC, G de Jager, and BT Sewell. 1997. "Use of a general imaging model to achieve predictive autofocus in the scanning electron microscope." Ultramicroscopy, 69 (1): 25-37.

Delpierre, GR and BT Sewell. 1995. "Designing for the real world: physical science courseware for South African schools." Spectrum, 33 (4): 18-20.

Parthasarathy, M, P Garcia-Lario, D de Martino, SR Pottasch, D Kilkenny, P Martinez, KC Sahu, BE Reddy, and BT Sewell. 1995. "Fading and variations in the spectrum of the central star of the very young planetary nebula SAO 244567 (Hen 1357)." Astronomy and Astrophysics, 300.

Sewell, BT and GR Delpierre. 1992. "Using computers to integrate, consolidate and improve biochemical education." Biochemical Education, 20 (1): 14-17.

Blundell, TL, JA Jenkins, BT Sewell, LH Pearl, JB Cooper, IJ Tickle, B Veerapandian, and SP Wood. 1990. "X-ray analyses of aspartic proteinases. The three-dimensional structure at 2.1 A resolution of endothiapepsin." Journal of Molecular Biology, 211 (4): 919-941.

Lawrence, MC, MA Jaffer, and BT Sewell. 1989. "The application of the maximum entropy method to electron microscopic tomography." Ultramicroscopy, 31 (3): 285-301.

von Holt, C, WF Brandt, HJ Greyling, GG Lindsey, JD Retief, JD Rodrigues, S Schwager, and BT Sewell. 1989. "Isolation and characterization of histones." Methods in Enzymology, 170, 431-523.

Greyling, HJ, BT Sewell, and C Von Holt. 1988. "The reconstitution of a hybrid histone octamer containing avian 110Cys-des-thio-histone H3 and sea-urchin 73Cys-histone H4." European Journal of Biochemistry, 171 (3): 721-726.

Landsman, D, BT Sewell, and C von Holt. 1988. "Aberrant DNase I digestion kinetics of nucleosomal core particles from sea urchin sperm." Biochemical and Biophysical Research Communications, 155 (1): 66-73.

Retief, JD, bt Sewell, and C Von Holt. 1987. "Nucleosome cores assembled in vitro occupy two preferred frames flanking the histone H1 gene from Psammechinus miliaris." Biochemistry, 26 (14): 4449-4453.

Brandt, WF, BT Sewell, and C von Holt. 1986. "Mr determination of histones from their electrophoretic mobility in acidic urea gels in the absence of detergents." FEBS Letters, 194 (2): 273-277.

Greyling, HJ, JP Hapgood, BT Sewell, and C von Holt. 1986. "Covalent labelling of histones with aurothiomalate. Gold-labelled H2A-H2B dimers assemble to octamers, which form crystalline helical tubes." European Journal of Biochemistry, 161 (1): 133-138.

Retief, JD, BT Sewell, HJ Greyling, S Schwager, and C von Holt. 1984. "Probing with DNase I of nucleosomal core particles assembled in vitro in the presence of polyglutamic acid." FEBS letters, 167 (1): 170-175.

Sewell, BT, C Bouloukos, and C von Holt. 1984. "Formaldehyde and glutaraldehyde in the fixation of chromatin for electron microscopy." Journal of Microscopy, 136 (Pt 1): 103-112.

Greyling, HJ, S Schwager, BT Sewell, and C von Holt. 1983. "The identity of conformational states of reconstituted and native histone octamers." European Journal of Biochemistry, 137 (1-2): 221-226.

Sewell, BT. 1982. "A rapid lookup table method for trigonometric functions." Software: Practice and Experience, 12 (10): 885-887.

Conferences and presentations

Sewell, BT. 2009. "The Nitrilases: Versatile Helix formers." Paper presented at the Gordon Research Conference on Three-Dimensional Electron Microscopy. 2009.

Kinfe, HH, VP Chhiba, J Frederick, K Mathiba, and D Brady. 2008. "Application of stereoselective biocatalysts for the enantiomeric resolution of beta-hydroxynitriles." Paper presented at the Biotechnology for the Sustainability of Human Society. 12-17 October 2008. Dalian, China. J Biotech. 136 (Supplement): S392.

Sewell, BT. 2007. "A national programme in structural biology." DST 2007.

Sewell, BT. 2007. "Sorghi summary.".

Sewell, BT. 2007. "The South African Structural Biology Initiative.".

Sewell, BT. 2007. "What are the connections between the helical interfaces and the active site in the nitrilases? Insights from the structure of the amidase from Geobacillus pallidus." GRC 2007.

Sewell, BT. 2007. "Why study nitrilases?".

Thuku, RN and BT Sewell. 2007. "The helical structure of the nitrilase from Rhodococcus rhodochrous J1 following a truncation in its C-terminus." Paper presented at the SPIDER Workshop.

Watermeyer, J and BT Sewell. 2007. "Domain-selective ACE inhibitors caught in the act.".

Abidemi, KM, NB Ndabambi, E AB, R Boelens, T Diercks, DJG Rees, and DJR Pugh. 2006. "Solution structure of the RING finger domain from the splicing-associated protein RbBP6 determined using heteronuclear Nuclear Magnetic Resonance (NMR) Spectroscopy." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Faro, A and DJR Pugh. 2006. "Characterization of the interaction between the p53 binding domain of the human RBBP6 protein and p53 using heteronuclear NMR." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Frederick, J, D Brady, and BT Sewell. 2006. "Enzymes: new tools for synthetic chemistry." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Kaunda, K, RA Atkinson, JC McKenzie, J Moolman-Smook, and DJR Pugh. 2006. "An investigation of protein-protein interaction involving cardiac Myosin Binding Protein C (cMyBP-C) using Nuclear Magnetic Resonance (NMR) spectroscopy." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Thuku, RN and BT Sewell. 2006. "The formation of helical oligomers in the nitrilase from Rhodococcus rhodochrous J1 following truncations in the C-terminal." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Woodward, JD, B Weber, M Scheffer, MJ Benedik, and BT Sewell. 2006. "Three-dimensional reconstruction of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Sewell, B. 2005. "Three dimensional electron microscopic imaging of chromatin." Paper presented at the SA Biochemical Society Ninth Congress. 2005. Wilderness, South Africa.

Thuku, RN and BT Sewell. 2005. "The quaternary structure of Rhodococcus rhodochrous J1 nitrilase as revealed by negative staining." Paper presented at the 44th Annual Meeting of the Microscopy Society of Southern Africa. 5–7 December 2005. Pietermaritzburg, South Africa. Proc Microsc Soc S Afr, 35: 31.

van Rooyen, JM, VR Abratt, and BT Sewell. 2005. "Three-dimensional structure of a type III glutamine synthetase by single-particle reconstruction." Paper presented at the 19th SASBMB Conference. 16-29 January 2005. Stellenbosch, South Africa. Proc S Afr Soc Biomol Molec Biol.

Varsani, A, MA Jaffer, A Williamson, and E Rybicki. 2002. "Deletion and point mutation products of the human papillomavirus type 16 major capsid gene." Paper presented at the 15th International Conference on Electron Microscopy. 103-104.

Sewell, B and M Jaffer. 2001. "Failure of a simple model to account for the iridescent colours of woodhoopoes." Paper presented at the Annual Meeting of the Electron Microscopy Society of South Africa. 2001. Johannesburg, South Africa. Proc Microsc Soc S Afr, 31: 82.

Buirski-Burger, N and BT Sewell. 1998. "Can computer based education save science education in South Africa." Paper presented at the Proc SAARMSE. 439-445.

Koch, I and T Lengauer. 1997. "Detection of distant structural similarities in a set of proteins using a fast graph-based method." Paper presented at the 5th International Conference on Intelligent Systems in Molecular Biology. 1997. Halkidiki, Greece. Proc Int Conf Intell Syst Mol Biol, Vol 5, pp. 167-178.

Nicolls, F, G de Jager, and BT Sewell. 1996. "A predictive autofocus algorithm for SEM." Paper presented at the Annual Meeting of the Microscopy Society of Southern Africa. 1996. Durban, South Africa. Proc Microsc Soc S Afr, 26: 5.

Jaffer, MA and BT Sewell. 1995. "Is a slow-scan CCD camera a viable alternative to film?" Paper presented at the Proc Electron Microsc Soc S Afr. 6.

Lipinski, MR, MD Durholtz, ME Waldron, Oosthuizen, J Erasmus, and BT Sewell. 1995. "The visualisation of growth increments." Paper presented at the Proc Electron Microsc Soc S Afr. 72.

Rost, B. 1995. "TOPITS: threading one-dimensional predictions into three-dimensional structures." Paper presented at the ISMB: International Conference on Intelligent Systems for Molecular Biology. Cambridge, UK. Proc Int Conf Intell Syst Mol Biol, Vol 3, pp 314-321.

Sewell, BT, VH Gartz, and HE Frimmel. 1995. "The technique of cathodoluminescence applied to Witwatersrand quartz using a Leica S440 and Oxford MonoCL." Paper presented at the Proc Electron Microsc Soc S Afr. 5.

Gerneke, DA and BT Sewell. 1994. "SEM and the digital paradigm - blessing or curse?" Paper presented at the Proc Electron Microsc Soc S Afr. 12.

Hubbard, TJP. 1994. "Use of β-strand interaction pseudo-potentials in protein structure prediction and modelling." Paper presented at the Twenty-Seventh Hawaii International Conference on System Sciences. 4-7 January 1994. Wailea, HI, USA. pp 336-344.

Knutsen, RD, BT Sewell, and DA Gerneke. 1994. "Electron backscatter diffraction analysis for microtexture determination." Paper presented at the Proc Electron Microsc Soc S Afr. 15.

Nicolls, FC, G de Jager, and BT Sewell. 1994. "Towards a predictive autofocus algorithm for SEM." Paper presented at the Annual Meeting of the Electron Microscopy Society of South Africa. 1994. Proc Electron Microsc Soc South Afr, 24: 11.

Clark, DA, CJ Rawlings, J Shirazi, A Veron, and M Reeve. 1993. "Protein topology prediction through parallel constraint logic programming." Paper presented at the ISMB: International Conference on Intelligent Systems for Molecular Biology. Bethesda, USA. Proc Int Conf Intell Syst Mol Biol, Vol 1, pp. 83-91.

Sewell, BT. 1993. "The three-dimensional structure of isolated chromatin fibres." Paper presented at the Proc Electron Microsc Soc S Afr. 43.

Graham, M, MA Jaffer, MF Calitz, and BT Sewell. 1992. "The diameter of chromatin fibres in sections of cell nuclei." Paper presented at the Proc Microsc Soc S Afr. 137-138.

Warren, R, C Von Holt, and BT Sewell. 1992. "Investigating the structure of material found in the Rancho La Brea Tar Pits, Los Angeles." Paper presented at the Proc Electron Microsc Soc S Afr. 19-20.

Jaffer, MA, M Graham, and BT Sewell. 1990. "Measurement of the diameters of chromatin fibres from chicken erythrocyte and sea urchin sperm." 185-186.

Jaffer, MA, M Graham, and BT Sewell. 1990. "The interpretation of stain density maps of chromatin fibres obtained by electron microscopic tomography." Paper presented at the Annual Meeting of the Electron Microscopy Society of Southern Africa. 1990. 151-152. Proc Electron Microsc Soc S Afr.

Sewell, B and MC Lawrence. 1986. "The structure of the chromatin fibre in sea urchin sperm." Paper presented at the Annual Meeting of the Electron Microscopy Society of SA Conference. 1986. Proc Electron Microsc Soc S Afr, 16: 49-50.

Lawson, T, B Sewell, and C Von Holt. 1984. "Measurement of nucleosome spacer lengths from dark field electron micrographs." Paper presented at the Annual Meeting of the Electron Microscopy Society of South Africa. 1984. Stellenbosch, South Africa. Proc Electron Microsc Soc S Afr, 14: 73-74.

Saffer, S and B Sewell. 1975. "A fibrous supporting structure in the human erythrocyte membrane." Paper presented at the Annual Meeting of the Electron Microscopy Society of South Africa. 1975. Proc Electron Microsc Soc South Afr, 5: 23-24.

Sewell, B. 1974. "A new theory for the structure of the human erythrocyte membrane." Paper presented at the Annual Meeting of the Electron Microscopy Society of South Africa. 1974. Proc Electron Microsc Soc S Afr, 4: 17-18.

Sewell, BT. "A personal view of structural biology in South Africa." University of Pretoria.

Book Sections

Tate, J. 2003. "Molecular Visualisation." In Structural Bioinformatics,, 135-158. Hoboken, New Jersey, USA: Wiley-Liss.

James, TL. 1998. "Fundamentals of NMR." In Biophysics Textbook Online,, Bethesda, MD: Biophysical Society, http://www.biophysics.org/btol/.

Slidel, TWF and JM Thornton. 1994. "Chirality in protein structure." In Protein folds. A distance based approach, edited by H Bohr and S Brunak, 253-264. New York: CRC Press.

Sewell, BT, GR Delpierre, and RC Lippert. 1993. "The CAI experience at UCT." In Computer-Based Education and Training in South Africa,, 51-66. Pretoria, South Africa: J.L. van Schaik Publishers.

Blundell, TL, J Jenkins, L Pearl, and BT Sewell. 1985. "The high resolution structure of endothiapepsin." In Aspartic Proteinases and their Inhibitors, edited by V Kostka, Berlin: Walter de Gruyter and Co.

Blundell, TL, J Jenkins, L Pearl, BT Sewell, and V Kostka. 1985. "The high resolution structure of Endothiapepsin in Aspartic Proteinases and their Inhibitors." Berlin.

Blundell, TL, T Sewell, W Turnell, G Dodson, JP Glusker, and D Sayre. 1981. "Symmetry in the structure and organization of proteins." In Structural Studies on Molecules of Biological Interest: A Volume in Honour of Professor Dorothy Hodgkin,, 390-403. Oxford, UK: Clarendon Press.

Blundell, TL, HB Jones, G Kahn, LH Pearl, T Sewell, G Taylor, and SP Wood. 1980. "The active site of acid proteinases." In Enzyme Regulation and Mechanisms of Action, edited by P Mildner and B Ries, 281-288. Oxford, UK: Pergamon Press Ltd.

Blundell, TL, T Sewell, JA Jenkins, IJ Tickle, C Slingsby, DS Moss, PF Lindley, WG Turnell, G Wistow, and R Srinivasan. 1979. "Structural evidence for gene duplication and fusion in proteins." In Biomolecular Structure, Conformation, Function and Evolution,, 251-256. Oxford, UK: Pergamon Press Ltd.

Blundell, TL, JA Jenkins, G Kahn, P Chowdhury, T Sewell, IJ Tickle, and EA Wood. 1979. "The three dimensional structure of acid proteinases." In Protein: Structure, Function and Industrial Applications, edited by E Hofmann, W Pfeil, and H Aurich, 81-94. Proceedings of the 12th FEBS meeting.

Blundell, TL, S Bedarkar, SP Wood, and T Sewell. 1977. "The evolution of the metal binding sites in proteins: Zinc binding of insulin and homologous proteins." In The Evolution of Metalloenzymes, Metalloproteins and Related Materials, edited by GJ Leigh, 1-16. London, UK: Symposium Press.

Jenkins, J, I Tickle, L Tickle, A Wollmer, T Sewell, and T Blundell. 1977. "X-ray analysis and circular dichroism of the acid proteases from Endothia Parasitica and Chymosin." In The Acid Proteases, edited by J Tang, 43-60. New York, USA: Plenum.

Layne, E. 1957. "[73] Spectrophotometric and turbidimetric methods for measuring proteins." In Methods in Enzymology,, 3, 447-454. Academic Press.

Other publications

Varsani, AD and EP Rybicki. 2006. "Chimaeric human papillomavirus 16 L1 virus like particles and a method for preparing the particles." University of Cape Town (Observatory, Cape Town, ZA), 2013/07/09/12:30:52.

Sewell, BT. 2005. "Treasure from the molecules of life." 2 (1): 30-33.

Delpierre, GR and BT Sewell. 2003. "A new web-based physical science resource for South African schools." Science in Africa, 2003.

Varsani, A and E Rybicki. 2003. "Chimeric human papillomavirus 16 L1 proteins comprising an L2 peptide,virus-like particles prepared therefrom and a method for preparing the particles." UNIVERSITY OF CAPE TOWN, VARSANI, Arvind, Devshi, RYBICKI, Edward, Peter, 2013/07/09/12:31:24.

Varsani, A, E Rybicki, and A-L Williamson. 2003. "Vectors, constructs, and transgenic plants for HPV-11 and HPV-16 L1 capsid protein." UNIVERSITY OF CAPE TOWN, VARSANI, Arvind, Devshi, RYBICKI, Edward, Peter, WILLIAMSON, Anna-Lise, 2013/07/09/12:30:57.

Sewell, BT. 1996. "Learning science - the future is here!" 38, 34-35.