SBRU publications

SBRU publications

Journal Articles

Crum, MA, JM Park, BT Sewell, and MJ Benedik. 2015. "C-terminal hybrid mutant of Bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization." Journal of Applied Microbiology, 118 (4): 881-889.

Crum, MA, JM Park, AE Mulelu, BT Sewell, and MJ Benedik. 2015. "Probing C-terminal interactions of the Pseudomonas stutzeri cyanide-degrading CynD protein." Applied Microbiology and Biotechnology, 99 (7): 3093-3102.

Ambele, MA, BT Sewell, FR Cummings, PJ Smith, and TJ Egan. 2013. "Synthetic hemozoin (beta-hematin) crystals nucleate at the surface of neutral lipid droplets that control their sizes." Crystal Growth & Design, 13 (10).

Weber, BW, SW Kimani, A Varsani, DA Cowan, R Hunter, GA Venter, JC Gumbart, and BT Sewell. 2013. "The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning." Journal of Biological Chemistry, 288 (40): 28514-28523.

Baum, S, DS Williamson, T Sewell, and A Stolz. 2012. "Conversion of sterically demanding alpha,alpha-disubstituted phenylacetonitriles by the arylacetonitrilase from Pseudomonas fluorescens EBC191." Applied and Environmental Microbiology, 78 (1): 48-57.

Broadley, SG, JC Gumbart, BW Weber, MJ Marakalala, DJ Steenkamp, and BT Sewell. 2012. "A new crystal form of MshB from Mycobacterium tuberculosis with glycerol and acetate in the active site suggests the catalytic mechanism." Acta Crystallographica. Section D, Biological crystallography, 68 (Pt 11): 1450-1459.

Manole, V, P Laurinmaki, W Van Wyngaardt, CA Potgieter, IM Wright, GJ Venter, AA van Dijk, BT Sewell, and SJ Butcher. 2012. "Structural insight into African horsesickness virus infection." Journal of Virology, 86 (15): 7858-7866.

Twala, BV, BT Sewell, and J Jordaan. 2012. "Immobilisation and characterisation of biocatalytic co-factor recycling enzymes, glucose dehydrogenase and NADH oxidase, on aldehyde functional ReSyn™ polymer microspheres." Enzyme and Microbial Technology, 50 (6-7): 331-336.

Wang, L, JM Watermeyer, AE Mulelu, BT Sewell, and MJ Benedik. 2012. "Engineering pH-tolerant mutants of a cyanide dihydratase." Applied Microbiology and Biotechnology, 94 (1): 131-140.

Chan, KY, J Gumbart, R McGreevy, JM Watermeyer, BT Sewell, and K Schulten. 2011. "Symmetry-restrained flexible fitting for symmetric EM maps." Structure (London, England: 1993), 19 (9): 1211-1218.

Miller, JL, J Woodward, S Chen, M Jaffer, B Weber, K Nagasaki, Y Tomaru, R Wepf, A Roseman, A Varsani, and T Sewell. 2011. "Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy." Journal of General Virology, 92 (8): 1960-1970.

Nel, AJM, IM Tuffin, BT Sewell, and DA Cowan. 2011. "Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate." Applied and Environmental Microbiology, 77 (11): 3696-3702.

van Rooyen, JM, VR Abratt, H Belrhali, and T Sewell. 2011. "Crystal structure of Type III glutamine synthetase: surprising reversal of the inter-ring interface." Structure (London, England: 1993), 19 (4): 471-483.

van Rooyen, J, H Belrhali, V Abratt, and BT Sewell. 2011. "Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements." Acta Crystallographica. Section F, Structural biology and crystallization communications, 67 (Pt 3): 358-363.

Achilonu, I, S Gildenhuys, L Fisher, J Burke, S Fanucchi, BT Sewell, M Fernandes, and HW Dirr. 2010. "The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function." Acta Crystallographica. Section F, Structural biology and crystallization communications, 66 (Pt 7): 776-780.

Gildenhuys, S, M Dobreva, N Kinsley, Y Sayed, J Burke, S Pelly, GP Gordon, M Sayed, BT Sewell, and HW Dirr. 2010. "Arginine 15 stabilizes an S(N)Ar reaction transition state and the binding of anionic ligands at the active site of human glutathione transferase A1-1." Biophysical Chemistry, 146 (2-3): 118-125.

Joubert, MK, N Kinsley, A Capovilla, BT Sewell, MS Jaffer, and M Khati. 2010. "A modeled structure of an aptamer-gp120 complex provides insight into the mechanism of HIV-1 neutralization." Biochemistry, 49 (28): 5880-5890.

Opperman, DJ, BT Sewell, D Litthauer, MN Isupov, JA Littlechild, and E van Heerden. 2010. "Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01." Biochemical and Biophysical Research Communications, 393 (3): 426-431.

van Rooyen, JM, VR Abratt, H Belrhali, and BT Sewell. 2010. "Crystallization of recombinant Bacteroides fragilis glutamine synthetase (GlnN) isolated using a novel and rapid purification protocol." Protein expression and purification, 74 (2): 211-216.

Watermeyer, JM, WL Kroger, HG O Neill, BT Sewell, and ED Sturrock. 2010. "Characterization of domain-selective inhibitor binding in angiotensin-converting enzyme using a novel derivative of lisinopril." Biochemical Journal, 428 (1): 67-74.

Williamson, DS, KC Dent, BW Weber, A Varsani, J Frederick, RN Thuku, RA Cameron, JH van Heerden, DA Cowan, and BT Sewell. 2010. "Structural and biochemical characterization of a nitrilase from the thermophilic bacterium, Geobacillus pallidus RAPc8." Applied Microbiology and Biotechnology, 88 (1): 143-153.

Woodward, JD and BT Sewell. 2010. "Tomography of asymmetric bulk specimens imaged by scanning electron microscopy." Ultramicroscopy, 110 (2): 170-175.

Dent, KC, BW Weber, MJ Benedik, and BT Sewell. 2009. "The cyanide hydratase from Neurospora crassa forms a helix which has a dimeric repeat." Applied Microbiology and Biotechnology, 82 (2): 271-278.

Frouws, TD, HG Patterton, and BT Sewell. 2009. "Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber." Biophysical Journal, 96 (8): 3363-3371.

Thuku, RN, D Brady, MJ Benedik, and BT Sewell. 2009. "Microbial nitrilases: versatile, spiral forming, industrial enzymes." Journal of Applied Microbiology, 106 (3): 703-727.

Woodward, JD, R Wepf, and BT Sewell. 2009. "Three-dimensional reconstruction of biological macromolecular complexes from in-lens scanning electron micrographs." Journal of Microscopy, 234 (3): 287-292.

Basile, LJ, RC Wilson, and BT Sewell. 2008. "Genome mining of cyanide-degrading nitrilases from filamentous fungi." Applied Microbiology and Biotechnology, 80 (3): 427-435.

Watermeyer, JM, WL Kroger, HG O Neill, BT Sewell, and ED Sturrock. 2008. "Probing the basis of domain-dependent inhibition using novel ketone inhibitors of angiotensin-converting enzyme." Biochemistry, 47 (22): 5942-5950.

Woodward, JD, BW Weber, MP Scheffer, MJ Benedik, A Hoenger, and BT Sewell. 2008. "Helical structure of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi." Journal of Structural Biology, 161 (2): 111-119.

Corradi, HR, I Chitapi, BT Sewell, D Georgiadis, V Dive, ED Sturrock, and KR Acharya. 2007. "The structure of testis angiotensin-converting enzyme in complex with the C domain-specific inhibitor RXPA380." Biochemistry, 46 (18): 5473-5478.

Danilov, SM, JM Watermeyer, IV Balyasnikova, K Gordon, EV Kugaevskaya, YE Elisseeva, RF Albrecht, and ED Sturrock. 2007. "Fine epitope mapping of monoclonal antibody 5F1 reveals anticatalytic activity toward the N domain of human angiotensin-converting enzyme." Biochemistry, 46 (31): 9019-9031.

Kimani, SW, VB Agarkar, DA Cowan, MFR Sayed, and BT Sewell. 2007. "Structure of an aliphatic amidase from Geobacillus pallidus RAPc8." Acta Crystallographica. Section D, Biological Crystallography, 63 (Pt 10): 1048-1058.

Makhongela, HS, AE Glowacka, VB Agarkar, BT Sewell, B Weber, RA Cameron, DA Cowan, and SG Burton. 2007. "A novel thermostable nitrilase superfamily amidase from Geobacillus pallidus showing acyl transfer activity." Applied Microbiology and Biotechnology, 75 (4): 801-811.

Thuku, RN, BW Weber, A Varsani, and BT Sewell. 2007. "Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form." FEBS Journal, 274 (8): 2099-2108.

AB, E, DJR Pugh, R Kaptein, R Boelens, and AMJJ Bonvin. 2006. "Direct use of unassigned resonances in NMR structure calculations with proxy residues." Journal of the American Chemical Society, 128 (23): 7566-7571.

Agarkar, VB, SW Kimani, DA Cowan, MFR Sayed, and BT Sewell. 2006. "The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing." Acta crystallographica. Section F, Structural biology and crystallization communications, 62 (Pt 12): 1174-1178.

Corradi, HR, AV Corrigall, E Boix, CG Mohan, ED Sturrock, PN Meissner, and KR Acharya. 2006. "Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen." Journal of Biological Chemistry, 281 (50): 38625-38633.

Corradi, HR, SLU Schwager, AT Nchinda, ED Sturrock, and KR Acharya. 2006. "Crystal structure of the N domain of human somatic angiotensin I-converting enzyme provides a structural basis for domain-specific inhibitor design." Journal of Molecular Biology, 357 (3): 964-974.

Farias, SL, RA Sabatini, TC Sampaio, IY Hirata, MHS Cezari, MA Juliano, ED Sturrock, AK Carmona, and L Juliano. 2006. "Angiotensin I-converting enzyme inhibitor peptides derived from the endostatin-containing NC1 fragment of human collagen XVIII." Biological Chemistry, 387 (5): 611-616.

Nchinda, AT, K Chibale, P Redelinghuys, and ED Sturrock. 2006. "Synthesis and molecular modeling of a lisinopril-tryptophan analogue inhibitor of angiotensin I-converting enzyme." Bioorganic & Medicinal Chemistry Letters, 16 (17): 4616-4619.

van Rooyen, JM, VR Abratt, and BT Sewell. 2006. "Three-dimensional structure of a type III glutamine synthetase by single-particle reconstruction." Journal of Molecular Biology, 361 (4): 796-810.

Watermeyer, JM, BT Sewell, SL Schwager, R Natesh, HR Corradi, KR Acharya, and ED Sturrock. 2006. "Structure of testis ACE glycosylation mutants and evidence for conserved domain movement." Biochemistry, 45 (42): 12654-12663.

Balyasnikova, IV, ZL Sun, YV Berestetskaya, AJ Chubb, RF Albrecht, ED Sturrock, and SM Danilov. 2005. "Monoclonal antibodies 1B3 and 5C8 as probes for monitoring the integrity of the C-terminal end of soluble angiotensin-converting enzyme." Hybridoma (2005), 24 (1): 14-26.

Jandhyala, DM, RC Willson, BT Sewell, and MJ Benedik. 2005. "Comparison of cyanide-degrading nitrilases." Applied Microbiology and Biotechnology, 68 (3): 327-335.

Kuhnert, DC, Y Sayed, S Mosebi, M Sayed, BT Sewell, and HW Dirr. 2005. "Tertiary interactions stabilise the C-terminal region of human glutathione transferase A1-1: a crystallographic and calorimetric study." Journal of Molecular Biology, 349 (4): 825-838.

Sewell, BT, RN Thuku, X Zhang, and MJ Benedik. 2005. "Oligomeric structure of nitrilases: effect of mutating interfacial residues on activity." Annals of the New York Academy of Sciences, 1056, 153-159.

Chubb, AH, SLU Schwager, E van der Merwe, MRW Ehlers, and ED Sturrock. 2004. "Deletion of the cytoplasmic domain increases basal shedding of angiotensin-converting enzyme." Biochemical and Biophysical Research Communications, 314 (4): 971-975.

Natesh, R, SLU Schwager, HR Evans, ED Sturrock, and KR Acharya. 2004. "Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme." Biochemistry, 43 (27): 8718-8724.

Sturrock, ED, R Natesh, JM van Rooyen, and KR Acharya. 2004. "Structure of angiotensin I-converting enzyme." Cellular and Molecular Life Sciences, 61 (21): 2677-2686.

Sturrock, ED, R Natesh, JM van Rooyen, and KR Acharya. 2004. "What's new in the renin-angiotensin system?" Cellular and Molecular Life Sciences, 61 (21): 2677-2686.

Acharya, KR, ED Sturrock, JF Riordan, and MRW Ehlers. 2003. "ACE revisited: a new target for structure-based drug design." Nature Reviews. Drug Discovery, 2 (11): 891-902.

Gordon, K, P Redelinghuys, SLU Schwager, MRW Ehlers, AC Papageorgiou, R Natesh, KR Acharya, and ED Sturrock. 2003. "Deglycosylation, processing and crystallization of human testis angiotensin-converting enzyme." The Biochemical journal, 371 (Pt 2): 437-442.

Guy, JL, RM Jackson, KR Acharya, ED Sturrock, NM Hooper, and AJ Turner. 2003. "Angiotensin-converting enzyme-2 (ACE2): comparative modeling of the active site, specificity requirements, and chloride dependence." Biochemistry, 42 (45): 13185-13192.

Jandhyala, D, M Berman, PR Meyers, BT Sewell, RC Willson, and MJ Benedik. 2003. "CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies." Applied and Environmental Microbiology, 39 (8): 4794-4805.

Natesh, R, SLU Schwager, ED Sturrock, and KR Acharya. 2003. "Crystal structure of the human angiotensin-converting enzyme-lisinopril complex." Nature, 421 (6922): 551-554.

Conferences and Presentations

Sewell, BT. 2009. "The Nitrilases: Versatile Helix formers." Paper presented at the Gordon Research Conference on Three-Dimensional Electron Microscopy. 2009.

Kinfe, HH, VP Chhiba, J Frederick, K Mathiba, and D Brady. 2008. "Application of stereoselective biocatalysts for the enantiomeric resolution of beta-hydroxynitriles." Paper presented at the Biotechnology for the Sustainability of Human Society. 12-17 October 2008. Dalian, China. J Biotech. 136 (Supplement): S392.

Sewell, BT. 2007. "A national programme in structural biology." DST 2007.

Sewell, BT. 2007. "Sorghi summary.".

Sewell, BT. 2007. "The South African Structural Biology Initiative.".

Sewell, BT. 2007. "What are the connections between the helical interfaces and the active site in the nitrilases? Insights from the structure of the amidase from Geobacillus pallidus." GRC 2007.

Sewell, BT. 2007. "Why study nitrilases?".

Watermeyer, J and BT Sewell. 2007. "Domain-selective ACE inhibitors caught in the act.".

Abidemi, KM, NB Ndabambi, E AB, R Boelens, T Diercks, DJG Rees, and DJR Pugh. 2006. "Solution structure of the RING finger domain from the splicing-associated protein RbBP6 determined using heteronuclear Nuclear Magnetic Resonance (NMR) Spectroscopy." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Faro, A and DJR Pugh. 2006. "Characterization of the interaction between the p53 binding domain of the human RBBP6 protein and p53 using heteronuclear NMR." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Frederick, J, D Brady, and BT Sewell. 2006. "Enzymes: new tools for synthetic chemistry." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Kaunda, K, RA Atkinson, JC McKenzie, J Moolman-Smook, and DJR Pugh. 2006. "An investigation of protein-protein interaction involving cardiac Myosin Binding Protein C (cMyBP-C) using Nuclear Magnetic Resonance (NMR) spectroscopy." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Thuku, RN and BT Sewell. 2006. "The formation of helical oligomers in the nitrilase from Rhodococcus rhodochrous J1 following truncations in the C-terminal." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Woodward, JD, B Weber, M Scheffer, MJ Benedik, and BT Sewell. 2006. "Three-dimensional reconstruction of unidirectionally shadowed metal replicas of cyanide hydratase from Gloeocercospora sorghi." Paper presented at the 1st African Structural Biology Conference. 24-27 October 2006. Wilderness, South Africa.

Sewell, B. 2005. "Three dimensional electron microscopic imaging of chromatin." Paper presented at the SA Biochemical Society Ninth Congress. 2005. Wilderness, South Africa.

Thuku, RN and BT Sewell. 2005. "The quaternary structure of Rhodococcus rhodochrous J1 nitrilase as revealed by negative staining." Paper presented at the 44th Annual Meeting of the Microscopy Society of Southern Africa. 5–7 December 2005. Pietermaritzburg, South Africa. Proc Microsc Soc S Afr, 35: 31.

van Rooyen, JM, VR Abratt, and BT Sewell. 2005. "Three-dimensional structure of a type III glutamine synthetase by single-particle reconstruction." Paper presented at the 19th SASBMB Conference. 16-29 January 2005. Stellenbosch, South Africa. Proc S Afr Soc Biomol Molec Biol.

Varsani, A, MA Jaffer, A Williamson, and E Rybicki. 2002. "Deletion and point mutation products of the human papillomavirus type 16 major capsid gene." Paper presented at the 15th International Conference on Electron Microscopy. 103-104.

Sewell, BT. "A personal view of structural biology in South Africa." University of Pretoria.

Book Sections

Other publications

Delpierre, GR and BT Sewell. 2003. "A new web-based physical science resource for South African schools." Science in Africa, 2003.