Determination of the structure of the plant natiuretic peptide irPNP from Arabidopsis thaliana

Determination of the structure of the plant natiuretic peptide irPNP from <em>Arabidopsis thaliana</em>

Plant natiuretic peptides are peptide hormones involved in water and salt homeostasis in plants (see () or consult Chris Gehring for further details). irPNP from Arabidopsis has a molecular weight of approximately 10kDa, making it suitable for either NMR or X-ray. The structure is currently uncharacterised.

The protein has been recombinantly expressed as a GST fusion and shown to be biologically active, and some optimisation of expression and solubility conditions has been done. The project will involve scale-up of expression and preliminary NMR studies and crystallisation trials. Depending on the success of the former, NMR or X-ray data will be collected and the structure determined in collaboration with either David or Muhammed.

  1. Gehring, C. A., & Irving, H. R. (2003). Natriuretic peptides--a class of heterologous molecules in plants. Int J Biochem Cell Biol, 35(9), 1318-22. PMID: 12798346 ()

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