Biochemistry and Assays

Biochemistry and Assays

  • BCA assay

    The bicinchoninic acid assay (BCA assay), also known as the Smith assay, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL).

  • Bradford assay

    The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein.

  • Lowry assay

    The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques.

  • Nitrilase activity assay

    1. Picric acid colorimetric assay for the quantitative determination of cyanide - for activity assays of cyanide-degrading nitrilases.
    2. Indophenol blue method for the quantitative detection of ammonia/ammonium - for activity assays of nitrilases that hydrolyze higher nitriles (benzonitrile among others) resulting in the production of ammonia among other products.

  • Ultraviolet absorbance of aromatic amino acids

    To different degrees, all aromatic amino acids absorb ultraviolet light.