Syllabus for "Introduction to Electron Microscopy for Biologists"
This course is an introductory overview of capabilities of LM, SEM, TEM and Image Analysis aimed at biological and medical Honours students. The course usually last four days and includes the events listed below. However the course can readily be restructured to suit the needs of a particular group of students by the deletion or addition of material. The course can also be extended by the addition of "hands on" practical sessions for the students.
Online Lectures:
Introduction to Microscopy for Biologists
Intracellular localisation of biological molecules
The Scanning Electron Microscope (IOWA)
Confocal laser scanning microscopy
Day 1
Lecture: The Application of Microscopy in the Biological Sciences.
Demonstration 1: The light microscope, fluorescence microscopy, phase contrast.
Demonstration 2: Photomicrography.
Demonstration 3: The vacuum coating plant, the preparation of thin films, shadowing.
Day 2
Demonstration 4: Fixing, dehydrating, embedding and sectioning.
Lecture 3: The TEM, design features, operation, image recording, major accessories.
Demonstration 5: Negative staining.
Demonstration 6: The operation of the TEM.
Lecture 4: Three dimensional TEM.
Day 3
Lecture 5: Specimen preparation for the SEM.
Demonstration 7: The Critical Point Dryer.
Demonstration 8: The Sputter Coater.
Lecture 6: The SEM, Design features, operation, major accessories including energy dispersive analysis of x-rays.
Demonstration 9: The operation of the SEM.
Day 4
Lecture 7: Measurement, Image Analysis and Data Reduction.
Demonstration 10: The measuring microscope.
Demonstration 11: The data tablet.
Demonstration 12: The GENIAS computer programme.
Demonstration 13: Lotus 1-2-3.
Lecture 8: The Photographic Process and Darkroom Technique.
Demonstration 14: Developing films.
Demonstration 15: Printing.
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